THE EVALUATION OF BONE PROTEOME OF PALM VITAMIN E‐SUPPLEMENTATION IN OVARIECTOMISED RATS

Abstract

IntroductionOsteoporosis is a common disease characterized by decreased bone mineral density (BMD) and microarchitectural deterioration of bone structure. Supplementation of palm vitamin E for 10 months was able to prevent loss of bone density in ovariectomised female rats. The mechanism by which palm vitamin E exerts these effects is still unknown. One study has found that some proteins were overexpressed in the ovariectomised rats as opposed to the control rats.ObjectiveWe hypothesized that palm vitamin E may act by preventing the protein expression changes.MethodThe study was divided into five groups; ovaritectomised treated with Palm vitamin E, Olive oil, alpha tocopherol, sham and normal. Femoral bones were harvested for protein extraction. Two‐dimentional gel electrophoresis was performed and proteins were analyzed using software genomic solutions Investigator.Result and DiscussionDifferential protein expression profile reveals that about five (5) protein spots were overexpressed in ovariectomised group treated with olive oil compared the ovariectomised group treated with palm vitamin E. While about three (3) protein spots were not expressed in the ovariectomised group treated with olive oil and were expressed in the group treated with palm vitamin E. The differential protein expression may account for the mechanism through which palm vitamin E exert its anti‐osteoporotic effect. However more research is ongoing to identify the proteins of interest.