Abstract
Background: Mindium laevigatum (Vent.) Rech. F. plant grows in central part of Iran. And used by local people as medical plant. Objectives: The purpose of this study was to investigate the in vitro antibacterial, antifungal and antioxidant activities of the methanolic extracts of aerial and flower parts of plant. Materials and Methods: The leaves and stem and flower of bark from M. laevigatum were separately collected, air-dried and powdered. Then the plant species extracts were prepared with methanol, water 80:20 and two polar and non-polar subfractions were realized. The antioxidant activity was evaluated by scavenging the radicals 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), β-Carotene linoleic acid assay and reducing power methods. The antifungal and antibacterial evaluation was performed by disc diffusion and minimum inhibitory concentration methods. Results: The total phenolic analysis of subfractions found 182 ± 4.2 µg.gr-1 for polar and 158 ± 3.9 µg.gr-1 for non-polar extracts. The antifungal activity of the extracts against the various fungal varied from 14.0 to 34âmm. MIC values from 50 to 400 µg.mL-1 were satisfactory when compared with other plant products. The antibacterial results revealed that the subfraction extracts are mostly effective against Staphylococcus aureus. The antioxidant results showed polar subfraction has more activity against non-polar subfraction. Conclusion: These findings demonstrated that the extract of Mindium laevigatum has remarkable in vitro antifungal and antioxidant activity.
Highlights
The request for reduced use of the synthetic antioxidants such as butylated hydroxyl toluene (BHT) and butylated hydroxyl anisole (BHA) in the food industry has triggered the need for the development of alternative active compounds, which are harmless to the consumers and to the environment while being useful for the protection of foods from microorganisms and free radicals [12, 13]
The amounts of phenolic compounds in polar and nonpolar subfraction of Mindium laevigatum were measured by the Folin and Ciocalteu reagent and expressed as gallic acid equivalents
The high phenolic content in polar subfraction of methanol extract contributes to its increased antioxidant potential in comparison to non-polar subfraction
Summary
Objectives: The purpose of this study was to investigate the in vitro antibacterial, antifungal and antioxidant activities of the methanolic extracts of aerial and flower parts of plant. The plant species extracts were prepared with methanol, water 80:20 and two polar and non-polar subfractions were realized. No report exists regarding the antifungal, antibacterial and antioxidant activities of the solvent extract of Mindium laevigatum (Vent.) Rech. This study is divided into two main objectives as an investigation of antifungal and anti-microbial activity of the polar and non-polar sub-fractions of methanolic extracts of Mindium laevigatum (Vent.) Rech. F. by disc diffusion and MIC against some pathogenic fungi and bacterial, and the antioxidant activity evaluation of extracts by three separate methods, namely inhibition of free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene– linoleic acid system and reducing power
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