The ethylene response factor (ERF) genes from Tamarix hispida respond to salt, drought and ABA treatment

Abstract

The ethylene response factor (ERF) genes are a subfamily of transcription factors containing a single AP2/ERF domain, which play crucial roles in plant development and response to biotic and abiotic stresses. In the present study, 16 ERF genes (named ThERF1–ThERF16) were isolated from Tamarix hispida, and their expression in response to salt, drought and ABA treatment was investigated using real-time RT-PCR. The results showed that all 16 ERFs are expressed in root, stem and leaf tissues, but the individual transcript levels vary strongly in roots, stem or leaves. Among these ThERFs, ThERF1 is the most abundant in roots, stems and leaves, indicating that it may play a more important physiological role than the other ThERF genes. The 16 ThERF genes are all highly differentially expressed in leaves, stems or roots under salt and drought stress, demonstrating that they are involved in the salt and drought stress responses. Under ABA treatment, expression of the ThERF genes was mainly down-regulated in roots, but induced in leaves or stems, suggesting that they are also involved in the ABA stress response. These results strongly suggest that ThERFs are abiotic stress response genes that may play important roles in the stress tolerance of T. hispida.