Abstract
The estrogen receptor in rat liver cytosol was analyzed as the [3H]estradiol-receptor complex by isoelectric focusing in slabs of polyacrylamide gel. The complex focused at pH 6.4. The radioactive peak became sharper and the recovery of receptor improved from 67% to 83% after limited trypsin digestion of [3H]estradiol-labeled cytosol before analysis. Using this technique, the estrogen receptor in rat liver cytosol was shown to have the expected ligand specificity. The [3H]estradiolreceptor complex had a dissociation constant of 7.6 × 10−10 m. Both male and female rat liver contained estrogen receptor. However, analysis of the receptor in male liver cytosol was seriously disturbed by an unspecific estrogen binder focusing at pH 5.3. This peak was eliminated when labeling of the estrogen receptor was performed in the presence of 0.1 mM unlabeled 5aandrostane-3α,17β-diol. Using this modified technique, no significant difffcience in receptor concentration was found between male and female rat liver cytosols (...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
