Abstract
Abstract Rat brains, not frozen, were homogenized in saline solution containing physostigmine, and also cupric chloride to inhibit choline acetyl-transferase. When brains were homogenized for periods of up to 3 min, the amount of acetylcholine extracted was proportional to the duration of homogenization. After 3 min, there was no further significant increase in acetylcholine. The acetylcholine extracted in this way was termed “free”; whereas that remaining in the brain tissue and extracted by acid-ethanol solution was termed “bound”. The total amount obtained from each brain was not significantly different from the total amount extracted by a more conventional method from the brains of rats killed by rapid freezing in liquid air. This observation applied also to brains removed from animals during anaesthesia and convulsions.
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