Abstract
A colorimetric method for serum cholesterol estimation has been described. The extraction method of Abell et al., as modified by Anderson and keys, was adopted and the extracted cholesterol was determined by the colour reaction with mixture of acetic and sulphuric acids. The advantages of the method are: (1) The stability of the optical density over a period of hours, (2) the stability of the reagent for 2 or 3 days, (3) the high degree of reproducibility of the results, and (4) the concordance with the method of Anderson and keys using the colour reagent of Liebermann-Burchard and with that of Sperry and webb. A colour reaction obtained by the addition of colour reagent containing acetic acid and sulphuric acid to cholesterol and to cholesterol solutions has been studied, and the effect of ferric and ferrous iron on the colour formation was investigated. It was concluded that Fe 3+ in trace amounts was responsible for the efficacy of the colour reagent and that B.D.H. sulphuric acid (Analytical Grade) contained a suitable concentration.
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