Abstract
A kininogen resembling polymerized bradykininogen (Bradykininogen II, Habermann and Oken, 1961) has been isolated from sheep, human and cat plasma. Although electrophoretically pure, the kininogen is contaminated by a small amount of an active kallikrein. At pH 7.0 this kallikrein causes the development of pharmacological activity in solutions of the kininogen. Pharmacological activation of the kininogen is prevented by soya bean trypsin inhibitor. The activated kininogen contracts the isolated uterus rat and ileum; injected i.v. into anaesthetized guinea-pigs and rats it causes, respectively, bronchonstriction and hypotension, diuresis and natriuresis; injected i.p. into unanaesthetized rats it provokes an antidiuresis and sodium retention which is replaced by diuresis and natriuresis in neurohypophysectomized rats. These actions are bradykinin-like. Deliberate polymerization of the kininogen and disc electrophoresis make the assay of plasma concentrations of the protein generating this kininogen simple. Polymerized kininogen Brady kininogen II Kininogen estimation Sodium excretion Water excretion
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