The Establishment of the Immortalize Cell Bank of Different Chinese Ethnic Groups

Abstract

Objecfive To reserve different Chinese ethnic groups'genomes,to establish a National cell bank and DNA bank of different Chinese ethnic groups,and to provide enough DNA for permanent research.Methods According to principle of informed consent,blood samples from unrelated individuals of different Chinese ethnic groups were collected and transformed using Epstein-Barr virus(EBV) to establish immortalized cell lines.Results The immortalized cell bank of 47 populations from 70 Chinese ethnic groups had been established.including 3982 immortalized cell lines and 7210 DNA samples.The matured and sta-ble technology of transferring B lymphocytes to immortalized cell using EBV had also been established.Con-clusion The largest immortalized cell lines bank in China has been established and can meet permanent DNA needs for further research.Some of cell lines and DNA from the bank have been provided to establish national and international co-operations. Key words: Different Chinese ethnic groups; Immortalize cell lines bank; DNA