The establishment of Lewis lung carcinoma model which express green fluorescence protein constantly in C57BL/6 mice

Abstract

Objective The present study aims to establish an orthotopic Lewis lung carcinoma (LLC) model on C57BL/6 mice where the cancer cells could express green fluorescence protein (GFP) stably. Methods 1×103, 1×104 and 1×105 Lewis lung cancer cells which could express GFP constitutively were seeded in Matrigel, respectively.Then, these groups of Matigel were planted orthotopically on 6-8 weeks female C57BL/6 mice.Every 3 mice were sacrificed and analyzed every 7 days. The tumor growth and metastasis was monitored directly using laser diode pumped (LDP)470 nm bright blue flashlight and Sellstrom Z87 fluorescence goggles. The tumorigenic rate and tumor formation time were defined, which were further confirmed by pathological examination and electron microscope analysis. Results All animals survived after the orthotopic intrapulmonary implantation and in all three groups the Lewis lung cancer cells in the formed tumor express GFP stably.The tumorigenic rate was 25. 00%, 58. 33% and 91. 67%, respectively, which correlated with the planted dosage and reached the significance(P 0. 05). Conclusion The Lewis lung carcinoma model which constantly expresses GFP was successfully established, and this could filter potential drug candidates against lung cancer. Key words: Model,animal; Rapid screening; Green fluorescence protein; Lewis lung carcinoma