The establishment of biological reference intervals of neonatal immunologic function in 24-hour newborns in Guangxi Province

Abstract

Objective To establish biological reference intervals of neonatal T lymphocyte subsets and IgG, IgA, IgM levels in 24-hour newborns in Guangxi. Methods Maternal history and neonatal clinical data were evaluated and recorded.Venous blood samplings were collected within 24 hours of birth and were sent for testing in half an hour.The neonates were divided into the early-preterm, the late-preterm and the term neonates group, 110 cases for each group.The parturients were divided into Dexamethasone treatment group and without Dexamethasone treatment group.Data in neonates and the parturients and the sex were analyzed by SPSS 17.0 software and the biological refe-rence values were calculated. Results The two-sided reference intervals of 95% in the early-preterm group, the late-preterm group and the term neonates group were as follows: CD3+ : 52.07-88.92 g/L, 58.16-90.42 g/L, 56.15-95.67 g/L; CD4+ : 25.20-59.26 g/L, 31.27-72.91 g/L, 28.44-82.66 g/L; CD8+ : 7.30-36.26 g/L, 9.13-38.49 g/L, 11.09-48.99 g/L; CD4+ /CD8+ : 0.34-4.58, 0.34-4.58, 0.32-3.80; CD19+: 3.95-27.59 g/L, 4.04-30.94 g/L, 4.08-38.70 g/L; NK cell: 1.34-6.64 g/L, 2.88-8.92 g/L, 3.07-9.35 g/L; IgA: 0.000 4-0.039 6 g/L, 0.000 0-0.069 0 g/L, 0.000 0-0.069 0 g/L; IgM: 0.001 6-0.158 4 g/L, 0.020 0-0.140 0 g/L, 0.020 0-0.420 0 g/L; IgG: 3.22-10.98 g/L, 1.10-14.62 g/L, 5.00-13.66 g/L.Moreover the cases with Dexamethasone treatment were as follows: the late-preterm infants CD8+ 10.35-40.33 g/L, NK 3.10-9.46 g/L, term NK 6.60-9.50 g/L; those in without Dexamethasone treatment: the late-preterm infants CD8+ 8.42-34.96 g/L, NK 2.94-7.80 g/L, term NK 2.98-8.94 g/L; according to gender, the males in the late-preterm infants CD8+ 8.26-35.66 g/L, term CD3+ 51.90-92.94 g/L; females in the late-preterm infants CD8+ 11.08-40.68 g/L, term CD3+ 61.10-96.14 g/L. Conclusions Testing values of neonatal T lymphocyte subsets and IgG, IgA, IgM levels in 24-hour newborns in Guangxi disperse largely and show some differences among the early-preterm neonates, the late-preterm neonates and the term neonates, and maternal Dexamethasone treatment during pregnancy and gender play a role in neonatal immunity. Key words: Infant, newborn; Immunologic function; T lymphocyte subsets; Immunoglubulin