The establishment of a rapid propagation system of the petiole of Hedera helix as explants

Abstract

Hedera helix is broadly distributed and cultivated globally. Hedera helix is very famous as a medicinal and ornamental plant. It is a traditional edible herb, which is beneficial to the treatment of various diseases and disorders, including cough, cold, reheumatoid arthritis and bronchial disorder. In this study, the petiole of Hedera helix was used as explants to study the effect of sodium hypochlorite disinfection time on explants, and the effects of different ratios of various plant growth regulators on callus induction and bud differentiation of explants were studied through orthogonal experiments. The optimal plant growth regulator ratio in rooting culture was also studied. The results showed that the explants were best sterilized with 2% sodium hypochlorite for 10 min. The best medium for callus induction was MS + 3.0 mg/L6-BA + 1.0 mg/L NAA + 0.1 mg/L2,4-D.The best bud differentiation medium was MS + 1.5 mg/L6-BA + 0.1 mg/L IBA + 0.5 mg/L TDZ. Significant results were achieved with rooting medium 1/2MS + 0.8 mg/L IBA. In conclusion, the objectives of the present investigation were to improve the disinfection procedure for petiole of Hedera helix explants using sterilization agents such as NaOCl with different time intervals. Moreover, the effects of different plant growth regulators on callus induction from petiole explants of Hedera helix were studied.