Abstract
BackgroundFilamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity. For now, a total of nine FtsH proteins have been encoded in rice, but their functions have not revealed in detail. In order to investigate the molecular mechanism of OsFtsH2 here, several osftsh2 knockout mutants were successfully generated by the CRISPR/Cas9 gene editing technology.ResultsAll the mutants exhibited a phenotype of striking albino leaf and could not survive through the stage of three leaves. OsFtsH2 was located in the chloroplast and preferentially expressed in green tissues. In addition, osftsh2 mutants could not form normal chloroplasts and had lost photosynthetic autotrophic capacity. RNA sequencing analysis indicated that many biological processes such as photosynthesis-related pathways and plant hormone signal transduction were significantly affected in osftsh2 mutants.ConclusionsOverall, the results suggested OsFtsH2 to be essential for chloroplast development in rice.
Highlights
Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity
Identification and sequence analysis of OsFtsH2 Among the nine members of the FtsH family identified in rice genome [28], sequence analysis showed that OsFtsH2 (LOC_Os01g43150) contained a complete ORF of 2031 bp and encoded a protein of 676 amino acids
A phylogenetic tree including ten FtsH2 proteins in rice and other plants was constructed (Fig. 1), and phylogenetic analysis showed that OsFtsH2 was clustered more closely with ZmFtsH2A and ZmFtsH2B than other FtsH2 proteins (Fig. 1A)
Summary
Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity. The development of chloroplast is a complex process modulated by both the plastid and nuclear genes, which can be divided into three steps [4]. The first step is to activate replication and DNA synthesis of plastid. The second step is Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease, which exists widely in eukaryotes (mitochondria and chloroplasts) and prokaryotes [8, 9]. It is a member of the AAA (ATPase associated with diverse cellular activities) protein family, and possesses ATPase activity, proteolysis activity and
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