Abstract
The Essential Role of Cytokinin Signaling in Root Apical Meristem Formation during Somatic Embryogenesis.
Highlights
Plant tissue culture and subsequent organogenesis have been regarded as part of the scientific roots of modern plant biotechnology (Sussex, 2008)
While auxin signaling cascade induces the expression of WUS (Su et al, 2011), little is known about the underlying mechanisms for the root apical meristem (RAM) establishment
Significantly decreased number of somatic embryos (SEs) and severely defected SEs were produced from embryonic callus of WUSCHEL-RELATED HOMEOBOX 5 (WOX5) antisense lines and plt2-1 mutant respectively, confirmed that WOX5 and PLT2 are required for somatic embryogenesis, and further indicated the role of WOX5 in the RAM is comparable to that of WUS in the shoot apical meristem (SAM)
Summary
Plant tissue culture and subsequent organogenesis have been regarded as part of the scientific roots of modern plant biotechnology (Sussex, 2008). A commentary on Establishment of embryonic shoot–root axis is involved in auxin and cytokinin response during Arabidopsis somatic embryogenesis by Su, Y. Establishments of shoot apical meristem (SAM) and root apical meristem (RAM) are critical steps for somatic embryogenesis (Scheres, 2007).
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