Abstract
The present study investigated the 1effects of both castration and manipulation of castrated1 subjects with 0.1 ml of 50µg/kg/day of testosterone at 3 weeks of age for 6 weeks on the pheromonal and histological structure of male mice preputial gland. The preputial gland results from the control intact group showed that six essential pheromonal compounds are present, whereas the glandular structure revealed well-developed acini with basement membrane and connective tissue around most of them. These findings also showed that castration has deleterious effects on the essential pheromonal compounds which are reduced to two compounds only (the α.-Farnesene and 1- Tetradecanol) as well as the histological sections from such subject appeared many atrophied vacuolated acini. On the other hand, treatment of castrated animals with such dose of testosterone did not show any marked effects on the essential pheromonal compounds but it was able to restore the activities of acinar development as showed by many hypertrophied alveoli.
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