The essential oil of Schinus terebinthifolius and its nanoemulsion and isolated monoterpenes: investigation of their activity against Culex pipiens with insights into the adverse effects on non-target organisms.

Abstract

The house mosquito, Culex pipiens L. is a harmful species, widespread in urban areas, and considered the primary enzootic vector of West Nile arbovirus. Widespread insecticide resistance in mosquito populations and the environmental risks and toxicity hazards of chemical pesticides make insecticides an inadequate mosquito control strategy. Seeking ecofriendly tools for mosquito control tools has become necessary. Essential oil (EO) was hydrodistilled from the fruits of Brazilian pepper, Schinus terebinthifolius Raddi and analyzed using gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. An oil-in-water nanoemulsion (particle size 41.3nm) was developed and characterized from EO using a green low-energy approach. EO, its nanoemulsion and monoterpenes showed mosquitocidal, repellent and acetylcholinesterase inhibitory activities against Cx. pipiens. A nanoemulsion concentration of 30 μl L-1 caused 100% larval mortality after 24 h of exposure, whereas EO, d-limonene and α-phellandrene at 60 μl L-1 caused 100%, 92.4% and 88.2% larval mortality, respectively. The concentration that killed 50% of organisms (LC50 ) for larvae after 24 h ranged between 6.8 and 40.6μl L-1 . Upon fumigation, 15.0μl L-1 of nanoemulsion killed 94.5% of adults after 24 h of exposure. LC50 values against adults ranged between 5.3 and 31.2μl L-1 . EO products exhibited repellence activity at concentrations between 0.5 and 4.0μl cm-2 . Test materials effectively inhibited the acetylcholinesterase activity of mosquito and were safe toward the non-target organisms Gambusia affinis and Eisenia fetida. There is a potential for using S. terebinthifolius EO, its nanoemulsion and monoterpenes as ecofriendly natural mosquitocides.