Abstract
Abstract The volatile shoot oil of Coleonema album was found to be composed of monoterpenes, sesquiterpenes and phenylpropanoids. Heterotrophic and photom ixotrophic callus cultures were established from green parts of the plant. A photom ixotrophic cell line contained a heterogeneous population of plastids and synthesized an essential oil, which differed qualitatively and quantitatively from the green parts of the intact plant. Substitution of 2,4-D and pCPA by a lower amount of NAA and high kinetin concentrations favoured the formation of essential oil. The heterotrophic callus, when grown on the same nutrient medium, was devoid of any differ entiated plastids and of essential oil constituents. Thus, a close correlation of light-induced plastid differentiation and essential oil synthesis was concluded.
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