Abstract
Cirrhosis of the liver often leads to edema and increased circulating and urinary levels of bile acids and bilirubin conjugates. We hypothesized that ENaC activation by increased concentrations of biliary metabolites in renal tubular ultrafiltrate could contribute to renal Na+ retention and edema in cirrhosis of the liver. The epithelial Na+ channel (ENaC) plays a key role in regulating Na+ reabsorption in the kidney, and is regulated by an array of factors, including extracellular ligands (e.g. H+, Na+, Cl−) and post‐translational modifications (e.g. cleavage and palmitoylation). To begin testing this, we expressed wild type and mutant ENaCs in Xenopus oocytes and measured effects on channel current by two‐electrode voltage clamp. We found that specific bile acids and conjugated‐bilirubin activate ENaC in a dose‐dependent manner, with deoxycholic acid having the strongest stimulatory effect. Notably, deoxycholic acid stimulated both wild type channels and ‘near silent’ channels in which the furin cleavage sites were mutated. However, fully activating channels by trypsin abolished deoxycholic acid stimulation in both wild type channels and channels lacking furin cleavage sites, consistent with an effect on open probability. We hypothesized that bile acids, conjugated bilirubin and Cys‐palmitoylation (palmitate addition to specific intracellular cysteines on the β and γ subunits) regulate ENaC currents through a common mechanism: by increasing the membrane association of specific intracellular structures, driving conformation changes in the channel's pore. To begin testing our hypothesis, we determined whether mutation of all 4 palmitoylation sites in the β and γ subunits (βC43, βC557, γC33 and γC41) to alanine would affect activation by deoxycholic acid. We found that deoxycholic acid stimulated currents of ENaC lacking of all 4 palmitoylation site cysteines by 7‐fold over wild type ENaC. When we tested the effect of mutating individual sites, we found that removal of the N‐terminal palmitoylation site cysteines resulted in the strongest stimulatory effects. We also found that deoxycholic acid had a stronger stimulatory effect when we removed both sites from the γ subunit as compared to the β subunit. Our results demonstrate that ENaC can be activated by biliary metabolites, including bile acids and bilirubin, and that these metabolites may activate ENaC through a mechanism in common with ENaC activation by palmitoylation.Support or Funding InformationDK098204 to O.B.K. and DK110332 to E.C.R.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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