Abstract
BackgroundEstimates of biological age derived from DNA-methylation patterns—known as the epigenetic clock—are associated with mortality, physical and cognitive function, and frailty, but little is known about their relationship with sedentary behavior or physical activity. We investigated the cross-sectional relationship between two such estimates of biological age and objectively measured sedentary and walking behavior in older people.MethodsParticipants were 248 members of the Lothian Birth Cohort 1936. At age 79 years, sedentary behavior and physical activity were measured over 7 days using an activPAL activity monitor. Biological age was estimated using two measures of DNA methylation-based age acceleration—i.e., extrinsic and intrinsic epigenetic age acceleration. We used linear regression to assess the relationship between these two estimates of biological age and average daily time spent sedentary, number of sit-to-stand transitions, and step count.ResultsOf the six associations examined, only two were statistically significant in initial models adjusted for age and sex alone. Greater extrinsic age acceleration was associated with taking fewer steps (regression coefficient (95% CI) − 0.100 (− 0.008, − 0.001), and greater intrinsic age acceleration was associated with making more sit-to-stand transitions (regression coefficient (95% CI) 0.006 (0.0001, 0.012). When we controlled for multiple statistical testing, neither of these associations survived correction (both P ≥ 0.17).ConclusionIn this cross-sectional study of 79-year-olds, we found no convincing evidence that biological age, as indexed by extrinsic or intrinsic epigenetic age acceleration, was associated with objectively measured sedentary or walking behavior.
Highlights
Prolonged sitting can increase risk of mortality and morbidity [1, 2]
There is consistent evidence that chronological age is strongly associated with deoxyribonucleic acid (DNA) methylation patterns [5, 6], and several DNA methylationbased biomarkers are used to estimate biological or “epigenetic age” [7, 8]
Limitations of this study were the lack of information on sedentary behavior and the use of self-reported physical activity data which could be subject to recall error and social desirability bias
Summary
Prolonged sitting can increase risk of mortality and morbidity [1, 2]. Being physically active lowers the risk of dying prematurely, but the effects of sedentary behavior seem to be independent of physical activity [1]. There is consistent evidence that chronological age is strongly associated with deoxyribonucleic acid (DNA) methylation patterns [5, 6], and several DNA methylationbased biomarkers are used to estimate biological or “epigenetic age” [7, 8] Such measures—often referred to as the “epigenetic clock”—are predictive of mortality independent of chronological age and other risk factors, supporting the notion that they capture some aspect of biological aging. The proportion of immune blood cell types changes with age [12, 13] These two epigenetic age measures were originally designed to assess whether including information about blood cell composition improved their ability to predict mortality [9]. We investigated the cross-sectional relationship between two such estimates of biological age and objectively measured sedentary and walking behavior in older people
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have