The epidemiological characteristics and molecular phylogeny of the dengue virus in Guangdong, China, 2015

Jiufeng Sun,Dawei Guan,Zhiqiang Peng,Jianfeng He,Huiqiong Zhou,Jinyan Lin,Qiqi Tan,Changwen Ke,Xin Zhang,Huan Zhang,Wenjun Ma,De Wu,Tao Liu,Songwu Cai,Jinhua Duan

doi:10.1038/s41598-018-28349-2

Jiufeng Sun, Dawei Guan + Show 13 more

Open Access

https://doi.org/10.1038/s41598-018-28349-2

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Abstract

In 2015, an unexpected multiple outbreak of dengue occurred in Guangdong, China. In total, 1,699 cases were reported, of which 1,627 cases were verified to have DENV infections by nucleic acid or NS1 protein, including 44 DENV-1, 1126 DENV-2, 18 DENV-3 and 6 DENV-4, and the other cases were confirmed by NS1 ELISA. Phylogenetic analyses of DENV-1 isolates identified two genotypes (I and V). The predominant DENV-2 outbreak isolates were the Cosmopolitan genotypes, which likely originated from Malaysia. The DENV-3 isolates were assigned into genotype I and genotype III. All 6 DENV-4 isolates from imported cases were likely originally from Cambodia, Thailand and the Philippines. The entomological surveillance showed a moderate risk for the BI index in Chaozhou and Foshan and a low risk in Guangzhou. The imported cases were mostly detected in Guangzhou and Foshan. Surprisingly, the most serious outbreak occurred in Chaozhou, but not in Guangzhou or Foshan. A combined analyses demonstrated the multiple geographical origins of this outbreak, and highlight the detection of suspected cases after the alerting of imported cases, early implementation of control policies and reinforce the vector surveillance strategies were the key points in the chain of prevention and control of dengue epidemics.

Highlights

Dengue virus (DENV) is an enveloped flavivirus of the family Flaviviridae and genus Flavivirus
Twenty-six and sixty-one isolates were randomly selected as representative of DENV-1 and -2, respectively, while all of the DENV-3 to -4 isolates were sent for E gene sequencing, resulting in 108 E gene sequences (DENV-1, 26; DENV-2, 61; DENV-3, 15; DENV-4, 6) (Fig. S2)
In 2010, imported Chikungunya virus caused an outbreak in a single city of Guangdong, China, but no continuous circulation of the virus was observed after the outbreak[11]

Summary

Introduction

Dengue virus (DENV) is an enveloped flavivirus of the family Flaviviridae and genus Flavivirus. The epidemiology, vector surveillance, control strategies and molecular characteristics of the DENV isolates involved in three cities (Guangzhou, Chaozhou and Foshan) were investigated to elucidate the potential gaps in the existing dengue prevention and control system of Guangdong, China.

Results

Conclusion