Abstract

The composition of alginates and their contribution to the dry weight of the lamina and stipe of Durvillaea potatorum have been examined. Assays of tissue samples were performed using the carbazole method and standards for these assays were prepared by partial acid hydrolysis of extracted alginates followed by differential precipitation of the fractions. The compositions of the standards were determined using high field I3C NMR spectroscopy. The lamina and stipe contain alginates rich in mannuronic acid, whereas the holdfast tissue is rich in guluronic acid. It is proposed that this variation in alginate composition is related to the function of the tissues in terms of providing flexibility for the lamina and stipe, and rigidity to the holdfast.

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