The environmental risk assessment of constructed wetlands filled with iron and manganese ores in typical antibiotic treatment

Abstract

Constructed wetlands (CWs) is considered as an efficient and environmentally friendly technology for advanced wastewater treatment to eliminate organic pollutants such as sulfamethoxazole (SMX) and trimethoprim (TMP). Iron (Fe) and manganese (Mn) ores have attracted more and more attention as CWs substrates in treating SMX and TMP, but the potentially negative environmental effects of wetland effluents, ore contaminants leached from the substrates and the risk of transmission of antibiotic resistance genes (ARGs) are still not clear. Three CW groups with different substrates (river sand (C-CW), Fe ore (Fe-CW), and Mn ore (Mn-CW)) were set up to evaluate the average removal rates and environmental risk in treating wastewater containing SMX and TMP. The results showed that the average removal rates of SMX and TMP by Fe-CW and Mn-CW were significantly higher than that of C-CW by 12.46%, 6.59% and 38.93%,15.39% respectively (p < 0.05), suggesting that both Fe and Mn ores facilitated the removal of antibiotics. However, the least abundance of ARGs was found in the layer of Fe ore at the middle layer (ML) in Fe-CW among all CWs, which suggested that Fe ore could reduce the risk of ARGs transmission. Although the environmental risk of Fe-CW and Mn-CW effluent was low, Fe-CW effluent inhibited the growth of Chlorella in both 48h and 72h experiments, while Mn-CW effluent showed an inhibitory effect in 48h and then promoted the growth in 72h. Meanwhile, these findings offer valuable insights for wetland health assessment and substrate selection for CWs.