Abstract

Environmental factors under which the stock (mother, donor) plant has been grown, including temperature; light duration, quality, and intensity; mineral nutrition; and ambient gases can have significant impact on explant responses in vitro. Bulblet production in hyacinth has been influenced by mother bulb storage temperature. Shoot proliferation and rootability of microshoots of rhododendron have been modified by light intensity and light quality. Photoperiod has been demonstrated to affect tuber and callus formation on potato and dahlia. Furthermore, plant growth regulator treatments of the stock plant can strongly influence in vitro responses of explants taken from such plants. Implications of these latter responses will be discussed with regard to possible endogenous hormonal activity.

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