Abstract
Two bioluminescence techniques (ATP and total adenylate assay) have been developed and used to enumerate airborne microorganisms collected under controlled conditions of temperature and humidity. The assays were applied to catches from two characterised bioaerosol sampling devices, the Aerojet-General glass cyclone and the IOM Personal Inhalable Aerosol Sampler. Measurement of the adenylate energy charge provided interesting insights into the metabolic state of the collected cells. The total adenylate method produced results in closer agreement with the ATP assay when using the glass cyclone. Results were obtained in 2.5 h and provide a useful method for area workplace monitoring. The application of the assays to a personal sampler have also been demonstrated. More developmental work is required to optimise the washing stages, and achieve similar benefits for the total adenylate assay.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
