Abstract
Abstract The enthalpy of hydrolysis of the 3'-bond of several cyclic 3',5'-nucleotides and of the 2'-bond of several cyclic 2',3'-nucleotides has been measured calorimetrically. In each case, the enthalpy was indicative of a high energy bond, although values for different compounds varied from -7.7 to -14.1 kcal mole-1. The enthalpy of hydrolysis of the cyclic 3',5'-nucleotides, but not that of the cyclic 2',3'-nucleotides, was markedly affected by the nature of the base.
Highlights
The enthalpy of hydrolysis of the 3’-bond of several cyclic 3’,5’-nucleotides and of the 2’-bond of several cyclic 2’,3’nucleotides has been measured calorimetrically
Enthalpies of hydrolysis were independent of nucleotide concentration, Amounts of enzyme used were sufficient in most cases to take the reaction to completion (>99%) within 10 min
The presence of unreacted cyclic nucleotides or the products of further breakdown would have been detected at a level of less than 1% of the total ultraviolet absorbing material
Summary
The enthalpy of hydrolysis of the 3’-bond of several cyclic 3’,5’-nucleotides and of the 2’-bond of several cyclic 2’,3’nucleotides has been measured calorimetrically. The enthalpy of hydrolysis of the cyclic 3’,5’-nucleotides, but not that of the cyclic 2’,3’-nucleotides, was markedly affected by the nature of the base. In the initial part of this study [6], it was reported that the enthalpy of hydrolysis of cyclic 3’) 5’-AMP at 25” is - 14.1 kcal mole-l and that that of cyclic 3’,5’-GMP is -10.5 kcal mole-l. Microcalorimetric techniques have been employed to measure the enthalpies of hydrolysis of the 3’-bond of cyclic inosine 3’ ,5’monophosphate, cyclic uridine 3’) 5’-monophosphate, cyclic 2’-deoxyribosyladenine 3’, 5’-monophosphate, and the 2’-bond of the cyclic 2’,3’-phosphates of adenosine, guanosine, cytosine, and uridine.
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