Abstract
The Drosophila melanogaster cell line 1182-4, which constitutively lacks centrioles, was established many years ago from haploid embryos laid by females homozygous for the maternal haploid (mh) mutation. This was the first clear example of animal cells regularly dividing in the absence of this organelle. However, the cause of the acentriolar nature of the 1182-4 cell line remained unclear and could not be clearly assigned to a particular genetic event. Here, we detail historically the longstanding mystery of the lack of centrioles in this Drosophila cell line. Recent advances, such as the characterization of the mh gene and the genomic analysis of 1182-4 cells, allow now a better understanding of the physiology of these cells. By combining these new data, we propose three reasonable hypotheses of the genesis of this remarkable phenotype.
Highlights
The centriole has likely been the subject of more controversy among cell biologists than any other organelle
One possibility is that the cell type that the 1182-4 cell line is derived from is susceptible to centriole loss when Spartan activity is impaired, whereas the other cell types that we examined are not
We examined the sequences of the core proteins involved in centriole biogenesis listed in Table 1, using the available sequence data for 1182-4 from the ModENCODE project [49]
Summary
The centriole has likely been the subject of more controversy among cell biologists than any other organelle. The first example of an established animal cell line constitutively lacking centrioles was the. This has been firmly established by two extensive ultrastructural studies [15,16], the origin of this remarkable phenotype has remained elusive for many years. The 1182 mutated gene (renamed “maternal haploid (mh)”) has been characterized [17,18]. This allows us to re-examine this “cold case” and re-start the work where it left off. We review our current understanding of the maternal haploid “saga” from a historical perspective to explore the topic, connecting the original findings on the 1182-4 cell line with our understandings of the mh mutant that initiated the investigation. We conclude by discussing the possible explanations of the intriguing disappearance of centrioles in this cell line and aim to provide more clues to solve this longstanding mystery
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