Abstract
Volvariella volvacea, also known as straw mushroom, is a common edible mushroom in Chinese cuisine. It contains many nutrients for human health. A fungal immunomodulatory protein (FIP) has been isolated from V. volvacea and named FIP-vvo. Although the regulatory effects of many FIPs on immunity have been identified, the impact of FIP-vvo in modulating dendritic cells (DCs), which play a key role to connect the innate and the adaptive immunity, is not known. In this study, we aim to study the effect of FIP-vvo on the DC maturation and function. We found that FIP-vvo slightly increased the generation of CD11c+ bone marrow-derived DC (BMDC). In addition, the surface expression of MHCII was promoted in BMDCs after the treatment of FIP-vvo, suggesting that FIP-vvo induces DC maturation. Furthermore, FIP-vvo enhanced the ability of BMDCs to activate antigen-specific T cell responses in vitro. In the in vivo study, the FIP-vvo treatment facilitated T cell response in lymph nodes. Therefore, for the first time, our data demonstrated that FIP-vvo promoted DC maturation and function and suggested that FIP-vvo could have benefits for human health by enhancing immunity.
Highlights
Mushrooms comprise a number of edible fungi and have been included in meals daily around the globe due to their high nutritional and functional properties [1]
We found that fungal immunomodulatory protein (FIP)-vvo promotes dendritic cells (DCs) maturation and enhances T cell activation
DCs were treated with various concentrations of FIP-vvo for 24 h, and the apoptosis was examined using Annexin V
Summary
Mushrooms comprise a number of edible fungi and have been included in meals daily around the globe due to their high nutritional and functional properties [1]. Volvariella volvacea, known as paddy straw mushroom or straw mushroom, is commonly used in Chinese cuisine [2]. It belongs to the saprophytic fungus in the Pluteaceae family and Basidiomycota phylum with high nutritional components and unique taste, and there are plenty in the tropical and subtropical regions of Southeast Asia. V. volvacea is an important source for healthy foods and medicines to regulate immune responses. Some types of lectin isolated from V. volvacea can potentially stimulate lymphocyte proliferation via the increasing calcium influx [3]. The protein fractions from V. volvacea have been reported to promote cytokine production by peripheral blood
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
