The endophytic bacterial entomopathogen Serratia marcescens promotes plant growth and improves resistance against Nilaparvata lugens in rice

Abstract

Entomopathogenic bacteria are commonly used as biological agents to control different insect pests. However, little is known about the role of bacterial entomopathogens as endophytes in regulating both plant growth and resistance against insect pests. Here, we applied the entomopathogenic bacterium Serratia marcescens S-JS1 via rice seed inoculation and evaluated its effects on host plant growth and resistance against the rice pest Nilaparvata lugens. Furthermore, the induction of defense-related secondary metabolites by the bacterium was assessed by GC–MS/MS. We showed that S-JS1 was able to endophytically colonize the roots and shoots of rice seedlings following seed inoculation. Colonized plants showed increased seed germination (9.4–13.3 %), root (8.2–36.4 %) and shoot lengths (4.1–22.3 %), and root (26.7–69.3 %) and shoot fresh weights (19.0–49.0 %) compared to plants without inoculation. We also identified the production of indole-3-acetic acid by S-JS1, which is likely involved in enhancing rice plant growth. In a two-choice test, N. lugens adults preferred to feed on untreated control plants than on plants treated with S-JS1. In the no-choice feeding tests, the survival of N. lugens nymphs that fed on S-JS1-treated plants was significantly lower than that of nymphs that fed on untreated plants. Additionally, seeds treated with 109 cfu/mL S-JS1 resulted in elevated levels of secondary metabolites, which may be associated with N. lugens resistance in rice plants. Therefore, we suggest that the entomopathogenic bacterium S. marcescens be considered a potentially promising endophyte for use in an innovative strategy for the integrated management of insect pests.