Abstract
Background Simultaneous delivery of HIV-1 antigens and genes by a single nanoparticle will greatly aid in the development of effective HIV-1 vaccines. We have created a highly stable, empty bacteriophage T4 capsid shell which can be engineered both inside and out. The empty head can be filled with foreign DNA using a powerful DNA packaging machine. Its surface can be arrayed with proteins as fusions of the outer capsid proteins, Hoc (highly antigenic outer capsid protein) and Soc (small outer capsid protein). The 120 x 86 nm head has a capacity to accommodate 170-kb foreign DNA and 1025 molecules of proteins. Methods
Highlights
Simultaneous delivery of HIV-1 antigens and genes by a single nanoparticle will greatly aid in the development of effective HIV-1 vaccines
The empty head can be filled with foreign DNA using a powerful DNA packaging machine
Its surface can be arrayed with proteins as fusions of the outer capsid proteins, Hoc and Soc
Summary
Simultaneous delivery of HIV-1 antigens and genes by a single nanoparticle will greatly aid in the development of effective HIV-1 vaccines. The emptied bacteriophage T4 head: a novel nanoparticle vehicle to simultaneously deliver HIV-1 antigens and genes into dendritic cells From AIDS Vaccine 2012 Boston, MA, USA. Background Simultaneous delivery of HIV-1 antigens and genes by a single nanoparticle will greatly aid in the development of effective HIV-1 vaccines. We have created a highly stable, empty bacteriophage T4 capsid shell which can be engineered both inside and out.
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