The emergence of foot-and-mouth disease virus serotype O PanAsia-02 sub-lineage of Middle East-South Asian topotype in Bangladesh.

Md Hossen,K Nazir,Md Islam,Mohammad Khan,Md Rahman,Sultan Ahmed,Sheikh Sayem,Sukumar Saha

doi:10.5455/javar.2020.g429

Abstract

Objective:This research work was conducted for the molecular characterization of the circulating foot-and-mouth disease (FMD) virus in Bangladesh and revealed out their serotype.Materials and methods:The VP1 gene of six field isolates of FMD virus (FMDV) serotypes (two serotypes O, two serotypes A, and two serotypes Asia 1) was subjected for sequencing and phylogenetic analysis. Neighbor-joining trees were constructed by using the Molecular Evolutionary Genetics Analysis 6, having the field nucleotide sequences of FMDV and related sequences available in the GenBank.Results:The nucleotide sequences of the VP1 genes of serotypes O, A, and Asia-1 of the isolates revealed that overall isolates were 91%–100% similar to the isolates reported from Bangladesh and other neighboring countries. Among the isolates reported from Bangladesh, serotype O had 98%–100% identity, serotype A had 91%–100% identity, and serotype Asia-1 had 94%–100% identity. A phylogenetic analysis revealed that the FMDV serotype O PanAsia-02 sub-lineage was confirmed in Bangladesh under the Middle East–South Asian (ME-SA) topotype. On the other hand, we identified genotype VII (18) of Asia topotype (serotype A) and lineage C (serotype Asia-1).Conclusion:The FMDV serotype O PanAsia-02 sub-lineage was confirmed in Bangladesh under the ME-SA topotype for the first time. The extensive cross-border animal movement from neighboring countries may act as the source of diversified FMDV serotypes in Bangladesh.

Highlights

Foot-and-mouth disease (FMD) is an economically important and highly contagious viral disease of cloven-hoofed animals, including cattle, sheep, goats, and swine. [1]
A phylogenetic analysis revealed that the FMD virus (FMDV) serotype O PanAsia-02 sub-lineage was confirmed in Bangladesh under the Middle East–South Asian (ME-SA) topotype
We identified genotype VII (18) of Asia topotype and lineage C

Summary

Introduction

Foot-and-mouth disease (FMD) is an economically important and highly contagious viral disease of cloven-hoofed animals, including cattle, sheep, goats, and swine. [1]. Foot-and-mouth disease (FMD) is an economically important and highly contagious viral disease of cloven-hoofed animals, including cattle, sheep, goats, and swine. The disease is caused by the genus Apthovirus belonging to the family Picornaviridae [2]. This RNA virus has seven serotypes, namely, A, O, C, Asia-1, and South African Territories 1, 2, and 3. FMD virus (FMDV) is subdivided into several serotypes, and each serotype may contain several subtypes [3]. The VP1 gene is the dominant epitope of capsid protein VP1, which is used for the detection of FMDV serotypes, and targeted for vaccine development. Within each serotype of FMDV, there is considerable antigenic diversity, and anti-sera against one strain of a serotype may not recognize other strains of the same serotype [2]

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Conclusion