Abstract
Zinc oxide reduced graphene oxide (ZnO/rGO) composite was synthesized via hydrothermal method. The presence of ZnO composite on the rGO sheets was proved by Fourier Transform Infrared (FTIR). The FTIR used to determine the functional group from the graphite to graphene oxide via the oxidation process and reduction from graphene oxide to ZnO/rGO. The electrochemical performance of bare glassy carbon electrode (GCE) and modified ZnO/rGO/GCE were observed using cyclic voltammetry technique with a different scan rates. ZnO/rGO/GCE showed an excellent electrical conductivity and fast electron transfer rate compare with bare GCE. ZnO/rGO/GCE was then successfully tested on the stock solution with different uric acid (UA) concentrations ranging from 10 µM to 100 µM and on urine sample. The R2 = 0.9914 was obtained from the ZnO/rGO/GCE calibration curve on different concentration versus anodic peak current. The mean recovery obtained was 109% meanwhile limit of detection (LOD) of uric acid in urine sample is 8.5 × 10−6 M. The results showed that modified ZnO/rGO/GCE had a good detection limit and high sensitivity which suitable for detection of uric acid in urine for the analytical and clinical application in future.
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