Abstract
Three specific enzymes, amygdalin hydrolase, prunasin hydrolase and hydroxynitrile lyase have, been isolated by continuous electrophoresis in a free buffer film from a purified, lyophilized preparation of almond β-glucosidase. Amygdalin hydrolase and prunasin hydrolase, although quite specific to their natural substrates, also displayed some activity towards salicin and β-nitrophenyl-β- D-glucopyranoside.
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