The electrophoresis of the high-tyrosine proteins of keratins on cellulose acetate strips

Abstract

1. 1. A method is described for the electrophoresis of the high-tyrosine proteins of keratins on cellulose acetate which provides resolution comparable with the previous starch procedure. The new method uses much less protein (5 vs. 50 μg), is much quicker (1 · 5 hr vs. several days) and gives sharper and cleaner patterns than the starch gel procedure. 2. 2. The staining of proteins on cellulose acetate with Coomassie brilliant blue has been improved by the introduction of a one bath fixing and staining procedure together with a single bath for destaining. This now means that the fiftyfold increase in sensitivity of Coomassie blue relative to the commonly used Ponceau S stain can be achieved more easily.