Abstract
Tobacco mosaic virus was readily detected in the proteins of infected plants by two simple micro techniques, one using electrophoresis of the proteins on filter paper, the other by paper chromatography of the proteins in 40% alcohol in water. The virus protein spot can be located, cut out, the virus eluted, and used for testing the infectivity by inoculating healthy plants. The proteins of tobacco plants infected with cucumber mosaic virus and potato Y virus could also be distinguished from the proteins of healthy plants by electrophoresis on filter paper, but a separate virus protein component was more difficult to distinguish than in the case of tobacco mosaic virus. Emilia and N. rustica plants infected with tomato spotted wilt virus did not yield proteins distinguishable from the healthy plants by this method. The protein components of healthy and mealy bug-infested pineapple plants were also compared by electrophoresis on filter paper. The diseased plants contained more of one protein component than the healthy plants. The proteins in the leaves of normal pineapple plants, as shown by their electrophoretic pattern on filter paper, were quite different from the proteins of tobacco and Emilia leaves. A new method of purifying tobacco mosaic virus is described.
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