The electrocardiographic changes generated by centrally applied arachidonic acid in rats

Abstract

Arachidonic acid (AA) and its metabolites have multifunctional regulatory effects on the central nervous system. Our previous reports disclosed that centrally injected AA organized the cardiovascular system in normal or hypotensive conditions by regulating the central and peripheral mechanism. In the light of the knowledge of the potential cardiovascular effects of AA, the current study aimed to investigate the effects of intracerebroventricular (ICV) injected AA on the electrocardiography (ECG) of the anesthetized rats. The adult Sprague Dawley rats were anesthetized with ketamine and xylazine mixture (50 mg/kg and 20 mg/kg; i.m., respectively). Under the anesthesia, the guide cannula was inserted into the left lateral ventricle of the rats. The ECG traces obtained from the lead II were written by placing electrodes on the limbs of the rats. Centrally injected AA (150 μg; ICV) statistically significantly (p<0.05) caused to the lengthening of the ECG waves and intervals, resulting in a decrease in the heart rate of the rats without changing the ECG waveforms, the amplitude, and also the isoelectric line. The obtained results clearly show that centrally injection of AA caused the deceleration in the heart electrical activity. The deceleration in the electrical activity of the heart caused to show bradycardia in the rats by extending the duration of the ECG waves and intervals.