Abstract
A combination of cultural medium and nourishment providing a high yield of target glycated forms of recombinant darbepoetin alfa produced by CHO cell culture was selected. The influence of different substances—precursors of glycans in glycosylation reactions—on the yield of highly sialylated forms of recombinant darbepoetin alfa was researched. It was shown that the use of 20 mM N-acetyl-D-mannosamine is the most effective for an increase in the concentration of highly sialylated forms of the protein in cultural fluid.
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