The efficiency of bridging sheet recruitment determines HIV-1 R5 envelope sensitivity to soluble CD4 and macrophage tropism

O O'Connell,M Duenas-Decamp,Ed Anton,A Repik,S Lu,X Kong,D Corti,Mp Gonzalez-Perez,A Wallace,Jd Reeves,S Zolla-Pazner,B Quitadamo,S Wang,R Lin,Pr Clapham,P Peters

doi:10.1186/1742-4690-9-s2-p141

Abstract

Background HIV-1 R5 viruses vary extensively in their capacity to infect macrophages. R5 viruses that confer efficient infection of macrophages are able to exploit low levels of CD4 for infection and they predominate in brain tissue where macrophages are a major target for infection. HIV-1 R5 variants that are transmitted are generally non-macrophage-tropic. Here, we investigated the sensitivity of macrophage-tropic and non-macrophage-tropic R5 envelopes to neutralizing antibodies.

Highlights

HIV-1 R5 viruses vary extensively in their capacity to infect macrophages
R5 viruses that confer efficient infection of macrophages are able to exploit low levels of CD4 for infection and they predominate in brain tissue where macrophages are a major target for infection
We observed striking differences in the sensitivity of Env+ pseudovirions to soluble CD4 compared to neutralizing monoclonal antibodies that target the CD4 binding site

Summary

Introduction

HIV-1 R5 viruses vary extensively in their capacity to infect macrophages. R5 viruses that confer efficient infection of macrophages are able to exploit low levels of CD4 for infection and they predominate in brain tissue where macrophages are a major target for infection. The efficiency of bridging sheet recruitment determines HIV-1 R5 envelope sensitivity to soluble CD4 and macrophage tropism O O’Connell6, A Repik6, JD Reeves1, MP Gonzalez-Perez6, B Quitadamo6, M Duenas-Decamp6, P Peters6, R Lin2, ED Anton1, S Zolla-Pazner3, D Corti4, A Wallace6, S Wang6, X Kong5, S Lu6, PR Clapham6* From AIDS Vaccine 2012 Boston, MA, USA.

Results

Conclusion