Abstract
An inactivated iron regulating protein (IRP) vaccine containing nine Pasteurella haemolytica serotypes was used. Two groups of Awassi lambs were used during the experiment. One group of 10 lambs was vaccinated and the second unvaccinated group of 10 lambs considered as negative control. Blood samples were collected on day 0, 2 and 4 weeks (post-vaccination) and 4 weeks after a revaccination. Antibody titers against capsular antigen of P. haemolytica serotypes A1, A2, A6, A7 and T3 was evaluated for both groups of lambs by indirect haemagglutination test (IHT). Antibody titers against P. haemolytica A2 outer membrane IRPs and the 35 kDa proteins was evaluated by enzyme linked immunosorbent assay (ELISA) and by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Results showed that the vaccine used induced sero-conversion against the capsular antigen, major outer membrane proteins (OMPs) and IRPs of P. haemolytica in the vaccinated lambs. Results also showed that one inoculation of the vaccine was insufficient to induce high antibody titers.
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