Abstract
SUMMARY: The lysis of yeast protoplasts as a function of the tonicity and composition of the suspension medium was examined. The resistance of the protoplasts to lysis by osmotic shock was lowered by certain chelating agents and at low pH values. The effects of chelating agents on the lysis of the protoplasts were inhibited by K+, Na+, Mg2+ and spermidine. Chelating agents increased lysis only when the protoplasts were undergoing osmotic stress, and the cations did not influence lysis unless chelating agents were also present. The presence on the protoplast membrane of a cation-binding site which is involved in maintaining the structure of the membrane is inferred.
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