The effects of triptolide on inflammatory responses in endothelial cells

Abstract

It is widely held that endothelial dysfunction plays a crucial role in various pathological inflammatory conditions. Although triptolide, an active ingredient of Traditional Chinese Medicine Tripterygium wilfordii Hook.F., has been shown to possess anti‐inflammatory effects in vivo, little information is available that describes the effects of triptolide on inflammatory responses in endothelial cells (ECs). In this study, we determined whether triptolide suppresses inflammatory responses of ECs via inhibition of NF‐κB signaling. In human umbilical vein endothelial cells (HUVECs), treatment with lipopolysaccharide (LPS) enhanced cytokine and chemokine production, adhesion molecule expression, and monocyte adhesion. These effects were associated with increased activation of NF‐κB pathway. Furthermore, we found that pretreatment with triptolide dose‐dependently attenuated LPS‐induced actions. Our results indicate that triptolide suppresses the inflammatory responses of ECs via the inhibition of NF‐κB signaling. [This work was supported by the National Natural Science Foundation of China (No. 81673734) and Henan Provincial Science and Technique Foundation for International Cooperation Program (No. 162102410048)].Support or Funding InformationThis work was supported by the National Natural Science Foundation of China (No. 81673734) and Henan Provincial Science and Technique Foundation for International Cooperation Program (No. 162102410048)This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.