The Effects of Taheri Consciousness Fields on the Growth of Escherichia Coli BL21 and Absorption of Lead Heavy Metal by Saccharomyces Cerevisiae in Altered Gravity Conditions

Abstract

In previous experiments, the growth of different microorganisms under the influence of T-Consciousness Fields (TCFs) was investigated. These fields with non-material and non-energetic properties can have traceable effects on the system under study, and it has been determined that they are different from magnetic, electric, and gravity fields. This study aims to investigate the effects of T-Consciousness Fields on the growth of Escherichia coli (E. coli) strain BL21 as a prokaryotic model organism under microgravity (MG) conditions and also to investigate the bioabsorption behavior of the heavy metal lead by Saccharomyces Cerevisiae, a eukaryotic model, in MG and Earth’s gravity (1G). In these experiments, which were conducted separately, samples without the influence of T-Consciousness Fields were considered as controls, and the duration of the experiment was 24 hours. First, bacterial growth was evaluated by measuring optical density at 600 nm (OD600). The results showed that the microgravity environment increased the growth of bacteria by about 1.4% compared to the earth's gravity (1G). While under the influence of T-Consciousness Fields, no significant difference was observed between MG and 1G samples. In the second study, the inductively coupled plasma mass spectrometry (ICP-MS) technique was used to evaluate heavy metal absorption. The results showed that yeast was able to absorb heavy metal in both gravities and MG environment increasing the absorption significantly and about 13.5% compared to Earth’s gravity. Although the effect of TCFs on the MG sample was not statistically significant, it significantly reduced the absorption of lead metal by about 9% in the conditions of the earth's gravity. In conclusion, the microgravity environment increased the growth of this strain of E. coli bacteria, and the application of T-Consciousness Fields inhibited this growth induction. Also, these fields affected the eukaryote model differently in 1G and MG gravity. It is suggested to investigate the effect of TCFs on the behavior of prokaryotic and eukaryotic organisms under microgravity stress in further studies.