The effects of stromal cell-derived factor 1α in secondary neovascular glaucoma of proliferative diabetic retinopathy

Abstract

Objective To observe the effects of stromal cell-derived factor 1α (SDF-1α) in secondary neovascular glaucoma (NVG) of proliferative diabetic retinopathy (PDR). Methods The vitreous specimens from 25 PDR patients (31 eyes) were collected with 13 NVG eyes and non-NVG 18 eyes. The concentrations of SDF-1α and vascular endothelial growth factor (VEGF) in those specimens were measured by enzyme-linked immunosorbent assay (ELISA). Human umbilical vein endothelial cells (HUVEC) were treated by different concentrations of SDF-1αand vascular endothelial growth factor (VEGF) in vitro, and the formation of tube cavity-like structure, length of capillary-like structures and 5'-bromo-2'-deoxyuridine (BrdU) labeling of treated HUVEC were measured. Results The length of HUVEC tube-like and capillarylike structure formation in 10, 100, 1000 ng/ml SDF-1α and 10 ng/ml VEGF groups were longer than that in the control group, the differences were statistically significant (P＜0. 01). The A value of BrdU labeling of 10, 100, 1000 ng/ml SDF-1α and 10 ng/ml VEGF groups were increased than that in the control group,the differences were statistically significant (P＜0.01). The vitreous levels of SDF-1α and VEGF of NVG specimens were higher than those in the non-NVG group, the differences were statistically significant (P＜0.01). Conclusions SDF-1α may promote the migration and proliferation of vascular endothelium cells, and participate in the neovascularization process in NVG patients with PDR. Key words: Diabetic retinopathy/complication; Glaucoma, neovascular/pathology; Chemokine CXCL12; Vascular endothelial growth factors