THE EFFECTS OF SPERMINE ON NEUROGENESIS AND THE PASSIVE AVOIDANCE LEARNING AFTER TRANSIENT CEREBRAL ISCHEMIA IN THE CHICKS

Abstract

Event Abstract Back to Event THE EFFECTS OF SPERMINE ON NEUROGENESIS AND THE PASSIVE AVOIDANCE LEARNING AFTER TRANSIENT CEREBRAL ISCHEMIA IN THE CHICKS Ebru Ayrancı1 and Suleyman Kaplan1* 1 Ondokuz Mayis University, Turkey Süleyman Kaplan1, M. Emin Önger1, Ebru Ayrancı1, G. Ömür Deniz1, Mehtap Güney1, M. Önder Karayiğit2, Zuhal Altunkaynak1, Murat Yarım2, Cafer Marangoz3 1Department of Histology-Embryology, Medical Faculty, Ondokuz Mayıs University, Samsun, Turkey 2Department of Pathology, Veterinary Faculty, Ondokuz Mayıs University, Samsun, Turkey 3Department of Physiology, Medical Faculty, Ondokuz Mayıs University, Samsun, Turkey ABSTRACT Objective: The aim of this study is to investigate the effects of spermine on neurogenesis and the passive avoidance learning following transient cerebral ischemia in the chicks. The neuroprotective effect of spermine in hippocampus is searched morphologically and functionally. Materials and Methods: The study was composed of three groups of chicks: pure controls (n=20), sham group (n=20) and ischemia groups (n=40). Passive avoidance was thought to 10 chicks respectively in the control and PA(+) sham group. The other 10 chicks of this group were not thought passive avoidance PA(-). Twenty chicks in the experiment group were PA(+), other 20 chicks were PA(-). The ischemia group was received a 20 minute ischemia. The ischemia and spermine group 30 minutes before the ischemia, intraperitoneally 10 mg/kg/day spermine were received. After seven days of the ischemia, all animals were retested for recalling and than were sacrificed. The brains were fixed with buffered formalin. The series of brain sections were stained with crystal violet. After staining, total numbers of neurons were estimated stereologically in Cyresl violet stained sections. Results: The number of probably newly developed neurons and total neurons were estimated using stereological techniques. There were found significant differences between the control PA(+) and the ischemia PA(-) groups (p<0.05); the control PA(+) and the ischemia+spermine PA(+) groups (p<0.01); the sham PA(+) and the ischemia+spermine PA(+) groups (p<0.05); the sham PA(-) and ischemia+spermine PA(-) groups (p<0.05); ischemia PA(+) and ischemia+spermine PA(+ ) (p<0.05). Conclusion: In this study we investigated the effects of spermine on neurogenesis and the passive avoidance learning following transient cerebral ischemia in the chicks. The neuroprotective effect of spermine in hippocampus was observed morphologically with cell counting technique and functionally with passive avoidance evaluation. The dose of spermine used in this study has a neuroprotective effect on the hippocampus after transient cerebral ischemia. Keywords: Ischemia, Spermine, Learning, Stereology, chick. Conference: 4th Conference of the Mediterrarnean Neuroscience Society, Istanbul, Turkey, 30 Sep - 3 Oct, 2012. Presentation Type: Poster Presentation Topic: Abstracts Citation: Ayrancı E and Kaplan S (2013). THE EFFECTS OF SPERMINE ON NEUROGENESIS AND THE PASSIVE AVOIDANCE LEARNING AFTER TRANSIENT CEREBRAL ISCHEMIA IN THE CHICKS. Conference Abstract: 4th Conference of the Mediterrarnean Neuroscience Society. doi: 10.3389/conf.fnhum.2013.210.00005 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Jan 2013; Published Online: 11 Apr 2013. * Correspondence: Prof. Suleyman Kaplan, Ondokuz Mayis University, Samsun, Turkey, skaplanomu@yahoo.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ebru Ayrancı Suleyman Kaplan Google Ebru Ayrancı Suleyman Kaplan Google Scholar Ebru Ayrancı Suleyman Kaplan PubMed Ebru Ayrancı Suleyman Kaplan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.