Abstract
Objective Analyze the effects of the deposit time and temperature of serum samples to the fluorescent quantitative DNA of patients with hepatitis. Methods The serum samples of patients who got a positive test result by the DNA fluorescent quantitative in the PCR laboratory of Man Zhouli No.1 hospital from January 2015 to April 2015 are placed in room temperature and 4 ℃ for one day and seven days respectively.Quantitative detection of virus in the samples were evaluated by using RT-PCR. Results At the same deposit time, there were no difference of the virus’ DNA quantitative detection between the serum samples that were placed in room temperature and above 4 ℃.While, under the same deposit temperature, the serum samples of the virus’ DNA quantitative detection deposited for one day and seven days also have no difference. Conclusion The virus’ DNA content detection of the hepatitis patients’ serum samples was not impacted by deposit time and temperature in seven days. Key words: HBV-DNA; The preservation of the specimen; RT-PCR
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