Abstract

The online version contains supplementary material available at 10.1007/s11032-021-01256-7.

Highlights

  • Rice is the most important staple food crop that feeds more than half of the world’s population, and the breeding of high-yielding rice varieties is very important to meet the requirements of an increasing world population

  • In 2018, the isonuclear alloplasmic japonica lines were used as females in separate crosses with NILRf5, NILRf6, and PPLRf5+Rf6, resulting in a testcross population consisting of nine pairs of F1 hybrids for evaluating the fertility restoring ability of these two Rf genes in japonica rice lines

  • It is obvious that the effect of HL-type cytoplasmic male sterility (CMS) on pollen fate differs in the indica and japonica genetic backgrounds, which is consistent with our previous findings (Zhang et al 2016)

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Summary

Introduction

Rice is the most important staple food crop that feeds more than half of the world’s population, and the breeding of high-yielding rice varieties is very important to meet the requirements of an increasing world population. Rice yield has increased significantly with the introduction of semi-dwarf varieties and the exploitation of heterosis, and hybrid rice technology is considered to be a major strategy for increasing the yield potential of rice (Cheng et al 2007). Three-line and two-line systems are the main strategies for development of hybrid rice. The three-line hybrid breeding system is composed of a cytoplasmic male sterile (CMS) line, a maintainer line and a restorer line (Fujimura et al 1996; Yuan 1994).

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