The Effects of Remote Ischemic Preconditioning and N-Acetylcysteine with Remote Ischemic Preconditioning in Rat Hepatic Ischemia Reperfusion Injury Model

Ali Ihsan Uysal,Ensari Guneli,Belgin Unal Togrul,Elvan Ocmen,Mert Akan,Tuncay Kume,Uğur Koca,Sevda Ozkardesler,Bekir Ugur Ergur

doi:10.1155/2014/892704

Ali Ihsan Uysal, Ensari Guneli + Show 7 more

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https://doi.org/10.1155/2014/892704

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Abstract

Background. Remote ischemic preconditioning (RIP) and pharmacological preconditioning are the effective methods that can be used to prevent ischemia reperfusion (IR) injury. The aim of this study was to evaluate the effects of RIP and N-Acetylcysteine (NAC) with RIP in the rat hepatic IR injury model. Materials and Methods. 28 rats were divided into 4 groups. Group I (sham): only laparotomy was performed. Group II (IR): following 30 minutes of hepatic pedicle occlusion, 4 hours of reperfusion was performed. Group III (RIP + IR): following 3 cycles of RIP, hepatic IR was performed. Group IV (RIP + NAC + IR): following RIP and intraperitoneal administration of NAC (150 mg/kg), hepatic IR was performed. All the rats were sacrificed after blood samples were taken for the measurements of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels and liver was processed for conventional histopathology. Results. The hepatic histopathological injury scores of RIP + IR and RIP + NAC + IR groups were significantly lower than IR group (P = 0.006, P = 0.003, resp.). There were no significant differences in AST and ALT values between the IR, RIP + IR, and RIP + NAC + IR groups. Conclusions. In the present study, it was demonstrated histopathologically that RIP and RIP + NAC decreased hepatic IR injury significantly.

Highlights

Liver ischemia/reperfusion injury (IRI) may occur during surgery, like hepatectomy or transplantation, or systemic hypoxia, like respiratory or circulatory failure
The histopathological injury score (HHIS) of the sham operated group was significantly lower than the ischemia reperfusion (IR), Remote ischemic preconditioning (RIP) + IR, and RIP + NAC + IR groups
The scores were significantly higher in the IR group than in the RIP + IR, and RIP + NAC + IR groups

Summary

Introduction

Liver ischemia/reperfusion injury (IRI) may occur during surgery, like hepatectomy or transplantation, or systemic hypoxia, like respiratory or circulatory failure. During ischemia toxic oxygen radicals are produced in the tissues These oxygen and superoxide radicals can cause endothelial injury, an increase in microvascular permeability and tissue edema in the reperfusion period [2, 3]. The aim of this study was to evaluate the effects of RIP and N-Acetylcysteine (NAC) with RIP in the rat hepatic IR injury model. Group II (IR): following 30 minutes of hepatic pedicle occlusion, 4 hours of reperfusion was performed. Group III (RIP + IR): following 3 cycles of RIP, hepatic IR was performed. Group IV (RIP + NAC + IR): following RIP and intraperitoneal administration of NAC (150 mg/kg), hepatic IR was performed. It was demonstrated histopathologically that RIP and RIP + NAC decreased hepatic IR injury significantly

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Conclusion