The effects of pseudomonasaeruginosa mannose sensitive hamemagglutination vaccine on epithelial-mesenchymal transition of hepatocellular carcinoma

Abstract

Objective To investigate the effects of pseudomonasaeruginosa mannose sensitive hamemagglutination vaccine(PA-MSHA) on the epithelial-mesenchymal transition(EMT) of hepatocellular carcinoma(HCC). Methods MHCC97L and HepG2 cells were cultured and treated with different dose of PA - MSHA. After that, morphological changes of cells were observed under a light microscope. Immunofluorescence and Western blot analysis were used to evaluate the expression of EMT related genes and transcription factors. Immunohistochemistry and reverse transcription polymerase chain reaction(RT- PCR)analysis were used to evaluate the expression of E- cadherin,Snail and Slug in the tumor tissue after the mice were treated with PA- MSHA. Results Western blot and immunofluorescence analysis reveled that the expression of E-cadherin was significantly elevated while expression of Vimentin was significantly inhibited by PA- MSHA,in a dose dependent manner. Moreover, the expression of fibronectin, nuclear factor- kappa B(NF-κB),Snail and Slug were also inhibited by PA- MSHA, but theses effects of PA- MSHA were blocked by mannose. After PA- MSHA treatment, HCC cells have altered from irregular shape to epithelial cobblestone appearance.In vivo study, PA- MSHA also significantly increased the expression of E- cadherin and inhibited the expression of Snail mRNA and Slug mRNA. Conclusion PA-MSHA can significantly inhibit EMT of HCC in a mannose-mediated manner. Key words: Hepatocellular carcinoma; Pseudomonas aeruginosa; Mannose; Epithelialmesenchymal transition