The effects of protein deprivation on the nucleus locus coeruleus: A morphometric golgi study in rats of three age groups

Abstract

In a previous morphometric Golgi study of the nucleus locus coeruleus we identified in rats fed a 25% casein diet 3 cell types, fusiform, multipolar and ovoid, and compared their age-related changes from 30 to 90 days and 90 to 220 days. In the present study we investigated the effects of an 8% casein diet, initiated prenatally and continued postnatally in the pups, using the same morphometric parameters at the same 3 ages. In these rats the majority of significant age-related changes were in primary and secondary dendritic spine density. On all 3 cell types between 30 and 90 days there was a decreased spine density followed by an increase between 90 and 220 days. These age-related changes closely followed those in controls and, as a result, when the two diet groups were compared at each age they showed only 4 significant differences out of 18 comparisons. In control rats these age-related changes in dendritic spine density in the nucleus locus coeruleus were diametrically out-of-phase with those found in the nucleus raphe dorsalis in a previous study. This is consistent with the postulated mutual inhibitory relationship between the nucleus locus coeruleus and nucleus raphe dorsalis. Comparison of these same events in the nucleus locus coeruleus and nucleus raphe dorsalis in the 8% casein diet rats show no evidence of an out-of-phase relationship. Thus, the relationship between these two closely related nuclei appears to be fundamentally altered by the 8% casein diet. In these comparisons of dendritic spine density and other parameters the 8% casein deit rats have shown in both the nucleus locus coeruleus and nucleus raphe dorsalis deficits and increases as compared to controls. In contrast, in morphometric Golgi studies of pyramidal and Purkinje cells undernutrition generally results in deficits or little change in the various parameters. This suggests that the adaptation of the non-pyramidal neurons in the present study is different from that shown by pyramidal and Purkinje cells.