The effects of previous ovarian status on ovulation rate and early embryo development in response to superovulatory FSH treatments in sheep

Abstract

A total of 64 ewes was used to determine if the changes in superovulatory yields related to the ovarian status at the start of superovulatory treatment are due to differences in the population of gonadotrophin-responsive follicles, alterations in the processes of ovulation or transport of embryos from oviduct to uterus and/or developmental competence of the oocyte/embryo. Ovarian status at the start of a superovulatory FSH step-down treatment, administered coincidentally with a progestagen, was assessed by ultrasonography. On Day 4 after progestagen withdrawal, embryos were recovered from oviduct and their viability was determined by assessing development in vitro culture (IVC) until the hatched blastocyst stage. In all the ewes, the ovulation rate was related positively to the number of 2–3 mm follicles at first FSH injection ( P < 0.005). However, the total number of embryos and their viability were related to the more limited category of 3 mm follicles ( P < 0.05), whereas a higher degeneration rate was related to the number of 2 mm follicles. The presence of a corpus luteum (CL) at the start of superovulatory treatment exerted a protective effect on embryonic viability, decreasing the degeneration of embryos. On the other hand, the presence of a dominant follicle at first FSH dose affected the mean size of the pool of follicles responding to the superovulation treatment, because ovulation arose from 3 to 5 mm follicles in absence of large follicles ( P < 0.05), but from 2 to 3 mm follicles when large follicles were present ( P < 0.005), indicating atresia in medium sized follicles in the presence of a large follicle.