The effects of PGD 2 and 9-deoxy-Δ 9-PGD 2 on colony formation of murine osteosarcoma cells

Abstract

Effects of antineoplastic prostaglandins (PG), PGD 2 and 9-deoxy-Δ 9-PGD 2, on colony formation of cloned Dunn osteosarcoma (TA 102), normal Swiss 3T3 and V-79 cell lines were evaluated. PGD 2 significantly inhibited the colony formation of TA 102 cells in a dose-dependent manner at concentrations between 0.5 and 5 ug/ml. The IC 50 value was calculated to be 0.72 ug/ml. A dose-dependent inhibition of TA 102 colony formation was also observed with 9-deoxy-Δ 9-PGD 2 between 0.01 to 1 ug/ml, the IC 50 value being 0.22 ug/ml. These prostaglandins did not exert cytocidal effects in vitro on Swiss 3T3 cells at concentrations between 0.01 to 1 ug/ml. The two agents had no significant cytocidal effects on V-79 cells except for 9-deoxy-Δ 9-PGD 2 at a concentration of 5 ug/ml. These results suggest that PGD 2 and 9-deoxy-Δ 9-PGD 2 are considered to have cytocidal activity on Dunn osteosarcoma cells in dosages which do not affect non-malignant cells.