The effects of olfactory bulb removal on single-pellet skilled reaching task in rats.

Abstract

We focused on how the rat uses olfactory cues in a single-pellet reaching task, which is composed of three successive learned responses, Orient, Transport, and Withdrawal. Orient comprised: front wall detection, slot localisation, and nose poke until reach start. High-speed video-recording enabled us to describe the temporal features of this sequence in controls vs. 3-5 and 12-14days after bilateral bulbectomy in trials with (P trial) vs. without (no-P trial) pellet. In controls, the full sequence was complete in P trials, while it was interrupted after Orient in no P-trials. After bulbectomy, the full sequence was seen in both P and no-P trials at days 3-5 and 12-14 and there was an increase in Orient duration due to the increased time in slot/shelf localisation. Unlike in controls, in anosmic rats, the first nose contact with the front wall took place below the slot/shelf level, and the number of nose touches together with the number of whisker cycles was significantly higher at 3-5 but not at 12-14days. The relationship between nose touches and whisker cycles was linear in all experimental conditions. Bulbectomy resulted in no changes in the Transport duration or the time the paw spent out of the slot. These findings suggest that olfaction allows the animal to orient itself in pellet localisation, and offers insight into the contribution of olfaction during different stages of natural behaviour in skilled reaching task.